Bioenergetic status and hypoxia in Lewis lung carcinoma assassed by 31P NMR spectroscopy: correlation with tumor progression.

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dc.contributor.author Bubnovskaya L.
dc.contributor.author Mikhailenko V.
dc.contributor.author Kovelskaya A.
dc.contributor.author Osinsky S.
dc.date.accessioned 2018-06-05T12:48:49Z
dc.date.available 2018-06-05T12:48:49Z
dc.date.issued 2007
dc.identifier.udk http://reposit.uni-sport.edu.ua/handle/787878787/1411
dc.description.abstract Aim: Тo evaluate the hypoxia level and some indices of Lewis lung carcinoma energy metabolism by means of 31Р NMR spectroscopy in perchloric acid (PCA) tissue extracts during growth of primary tumor and metastasis. Мaterials and Methods: C57Bl/6 mice-bearing Lewis lung carcinoma were used in this study. Tumor energy metabolism was studied by 31P NMR spectroscopy and the metabolic NMR ratios were used as parameters for metabolic status and hypoxia level. Results: It was shown that growth of primary tumor is accompanied with increase of Pi/PCr, Pi/βNТP and PME/βNТP ratios that reflect drop of tumor energy status and oxygenation level in tumor tissue. These changes in relevant metabolic ratios correlate with enlargement of primary tumor volume (r = 0.87, p = 0.0045; r = 0.90, p = 0.0012; r = 0.764, p = 0.05, respectively) as well as with the umber of lung metastases (rs = 0.761, p = 0.028; rs = 0.86, p = 0.0049; rs = 0.77, p = 0.040, respectively). Conclusion: In present study it was shown that 31Р NMR spectroscopy of PCA tumor tissue extracts may be used as reliable method for the assessment of the level of oxygenation as well as changes in energy metabolism in the experimental tumors. It may be helpful to evaluate the energy status of human tumors by investigation both of biopsy and surgical specimens. Hypoxia and hypoxia-associated metabolic events in primary tumor are linked with malignant progression, in particular metastasis. uk_UA
dc.language.iso en uk_UA
dc.publisher Experimental Оncology uk_UA
dc.subject Bioenergetic status uk_UA
dc.subject Hypoxia uk_UA
dc.subject Lewis lung carcinoma (3LL) uk_UA
dc.subject 31P NMR spectroscopy uk_UA
dc.subject metastasis uk_UA
dc.title Bioenergetic status and hypoxia in Lewis lung carcinoma assassed by 31P NMR spectroscopy: correlation with tumor progression. uk_UA
dc.type Article uk_UA


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